UJI METODE INOKULASI DAN PATOGENISITAS BLOOD DISEASE BACTERIUM (BDB) PADA BUAH PISANG (Musa Sp.)

Abstract

This study aims to examine several inoculation methods for pathogenisity test of
BDB on banana fruit. The Results showed that injection method and smearing
suspension bacteria using cotton bud on the base of fruit was capable in producing
symptoms of blood disease on banana fruit. The injection method is shown to be
the most effective method because it caused blood disease symptoms in the
banana fruit at the first day after inoculation. Inoculums densities of 108 and 109
CFU/ml of BDB could develop disease on third and sixth day after inoculation.
BDB produced unique symptoms by causing yellowing and browning on the flesh
of a banana fruit as well as browning at the fruit placenta in 7 days after
inoculation, the symptom is different with caused by other pathogen like as
Xanthomonas oryzae, Pantoea sp dan Erwinia carotovora, dan Ralstonia
solanacearum.

Keywords: banana, Blood Disease Bacterium (BDB), inoculation